1AMX Bacterial Adhesin date Jun 19, 1997
title Collagen-Binding Domain From A Staphylococcus Aureus Adhesin
authors J.Symersky, S.Narayana
compound source
Molecule: Collagen Adhesin
Chain: A
Fragment: Cbd (151 - 318)
Synonym: Cbd19
Engineered: Yes
Other_details: A 19-Kda Fragment Of The Collagen-Binding Adhesin From A Staphylococcus Aureus (His-Tag + Residues 151-318)
Organism_scientific: Staphylococcus Aureus
Gene: Cna
Expression_system: Escherichia Coli
Expression_system_strain: Jm101
Expression_system_cellular_location: Cytoplasm
Expression_system_vector_type: Plasmid
Expression_system_vector: Pqe-30
Expression_system_plasmid: Pqe504
Expression_system_gene: Cna
symmetry Space Group: P 32 2 1
R_factor 0.200
crystal
cell
length a length b length c angle alpha angle beta angle gamma
74.000 74.000 56.700 90.00 90.00 120.00
method X-Ray Diffractionresolution 2.00 Å
note 1AMX is a representative structure
similarity Belongs to the Collagen_bind family.
subcellular loc. Attached to the cell wall peptidoglycan by an amide bond (potential).
Gene CNA (S. aureus)
function Mediates attachment of staphyococcal cells to collagen- containing substrata.
Gene
Ontology
ChainFunctionProcessComponent
A
  • binding
  • collagen binding

  • cell wall
  • cell surface
  • Primary referenceStructure of the collagen-binding domain from a Staphylococcus aureus adhesin., Symersky J, Patti JM, Carson M, House-Pompeo K, Teale M, Moore D, Jin L, Schneider A, DeLucas LJ, Hook M, Narayana SV, Nat Struct Biol 1997 Oct;4(10):833-8. PMID:9334749
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (40 Kb) [Save to disk]
  • Biological Unit Coordinates (1amx.pdb1.gz) 36 Kb
  • CSU: Contacts of Structural Units for 1AMX
  • Likely Quarternary Molecular Structure file(s) for 1AMX
  • Structure Factors (96 Kb)
  • Retrieve 1AMX in mmCIF format [Save to disk]
  • View 1AMX in 3D
  • Proteopedia, because life has more than 2D.
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1AMX
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electron Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1AMX, from MSDmotif at EBI
  • Genome occurence of 1AMX's fold from GeneCensus
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1amx__, region [Jmol] [rasmolscript] [script source]
  • Fold representative 1amx from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1amx_A]
  • Other resources with information on 1AMX
  • InterPro: IPR001899 , IPR008456 , IPR008454
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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