1AOD Hydrolase date Jul 02, 1997
title Phosphatidylinositol-Specific Phospholipase C From Listeria Monocytogenes
authors D.W.Heinz, J.Moser
compound source
Molecule: Phosphatidylinositol-Specific Phospholipase C
Chain: A
Synonym: Pi-Plc
Ec: 3.1.4.10
Engineered: Yes
Other_details: Bound Myo-Inositol In Active Site
Organism_scientific: Listeria Monocytogenes
Strain: Egd Serotype 12a
Cell_line: Bl21
Cellular_location: Secreted Into Medium
Gene: Plca
Expression_system: Escherichia Coli
Expression_system_strain: Bl21 (De3)
Expression_system_cellular_location: Cytoplasm
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Plca
Expression_system_gene: Plca Deletion Variant
symmetry Space Group: P 31 2 1
R_factor 0.158
crystal
cell
length a length b length c angle alpha angle beta angle gamma
82.100 82.100 92.100 90.00 90.00 120.00
method X-Ray Diffractionresolution 2.60 Å
ligand INS enzyme Lyase E.C.4.6.1.13 BRENDA
related structures by homologous chain: 2PLC
similarity Contains 1 pi-plc x-box domain.[PI-PLC-X]
subunit Monomer.
catalytic activ. 1-phosphatidyl-1d-myo-inositol = 1d-myo- inositol 1,2-cyclic phosphate + diacylglycerol.
subcellular loc. Membrane localization by OPM: Secreted
out side
in side
Genes LMO0201, PIC, PLCA (L. monocytogenes)
function Important factor in pathogenesis, pi-plc activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane. Cleaves glycosylphosphatidylinositol (gpi) and phosphatidylinositol (pi) anchors but not pi phosphates.
Gene
Ontology
ChainFunctionProcessComponent
A
  • phosphatidylinositol diacylg...
  • phospholipase C activity
  • lyase activity
  • signal transduction
  • intracellular signaling casc...
  • pathogenesis
  • lipid catabolic process
  • cytoplasm
  • Primary referenceCrystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes., Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW, J Mol Biol 1997 Oct 17;273(1):269-82. PMID:9367761
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (51 Kb) [Save to disk]
  • Biological Unit Coordinates (1aod.pdb1.gz) 47 Kb
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