1BY9	Transcription Regulation	date	Oct 27, 1998
title	Crystal Structure Of The E2 Dna-Binding Domain From Human Papillomavirus Type-16: Implications For Its Dna Binding- Site Selection Mechanism
authors	R.S.Hegde, E.J.Androphy
compound		source
Molecule: Regulatory Protein E2 Chain: A Fragment: Dna-Binding Domain Engineered: Yes		Organism_scientific: Human Papillomavirus Type 16 Cell_line: Bl21 Gene: E2 Expression_system: Escherichia Coli Expression_system_strain: Bl21 (De3) Expression_system_plasmid: Bl21
symmetry	Space Group: P 31 2 1	R_factor	0.195
crystal cell	length a length b length c angle alpha angle beta angle gamma 43.775 43.775 76.570 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.20 Å
note	1BY9 is a representative structure
related structures	by homologous chain: 1A7G
similarity	Belongs to the papillomaviruses e2 protein family.[PPV_E2_C]
subunit	Binds dna as a dimer.
subcellular loc.	Nucleus.
Gene	E2 (H. papillomavirus)
function	The e1-e2 complex binds to the origin of dna replication. It can either activate or repress transcription depending on e2re's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. E2 regulates viral transcription and dna replication. It binds to the e2re response element (5'-accnnnnnnggt-3') present in multiple copies in the regulatory region.
Gene Ontology	Chain Function Process Component A nucleotide binding DNA binding transcription factor activit... regulation of DNA replicatio... regulation of transcription,... viral reproduction host cell nucleus
Primary reference	Crystal structure of the E2 DNA-binding domain from human papillomavirus type 16: implications for its DNA binding-site selection mechanism., Hegde RS, Androphy EJ, J Mol Biol 1998 Dec 18;284(5):1479-89. PMID:9878365

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (16 Kb) [Save to disk] Biological Unit Coordinates (1by9.pdb1.gz) 24 Kb CSU: Contacts of Structural Units for 1BY9 Likely Quarternary Molecular Structure file(s) for 1BY9 Retrieve 1BY9 in mmCIF format [Save to disk]

View 1BY9 in 3D

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1BY9, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1by9__, region [Jmol] [rasmolscript] [script source] Fold representative 1by9 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1by9_A]

Other resources with information on 1BY9

InterPro: IPR001866 , IPR012677 , IPR000427 MMDB (Entrez's Structure Database)

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