1DI0	Transferase	date	Nov 28, 1999
title	Crystal Structure Of Lumazine Synthase From Brucella Abortus
authors	B.C.Braden, C.A.Velikovsky, A.A.Cauerhff, I.Polikarpov, F.A.Goldbaum
compound		source
Molecule: Lumazine Synthase Chain: A, B, C, D, E Engineered: Yes		Organism_scientific: Brucella Abortus Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria
symmetry	Space Group: H 3 2	R_factor	0.180
crystal cell	length a length b length c angle alpha angle beta angle gamma 142.250 142.250 242.300 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.70 Å
ligand	PO4	enzyme
note	1DI0 is a representative structure
related structures	by homologous chain: 1T13
similarity	Belongs to the DMRL_synthase family.
subunit	Homopentamer (probable).
catalytic activ.	2 6,7-dimethyl-8-(1-d-ribityl)lumazine = riboflavin + 4-(1-d-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
pathway	Riboflavin biosynthesis; last step.
Genes	BRUAB2 ; RIBH-2, RIBH2, RIBH (B. abortus)
function	The beta subunit catalyzes the condensation of 5-amino-6-(1'-d)-ribityl-amino- 2,4(1h,3h)-pyrimidinedione with l-3,4-dihydrohy-2-butanone-4- phosphate yielding 6,7-dimethyl-8-lumazine. Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-d)- ribityl-amino-2,4(1h,3h)-pyrimidinedione and l-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine.
Gene Ontology	Chain Function Process Component A, D, C, E, B riboflavin synthase activity... transferase activity riboflavin biosynthetic proc... riboflavin synthase complex
Primary reference	Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortus., Braden BC, Velikovsky CA, Cauerhff AA, Polikarpov I, Goldbaum FA, J Mol Biol 2000 Apr 14;297(5):1031-6. PMID:10764570

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (122 Kb) [Save to disk] Biological Unit Coordinates (1di0.pdb1.gz) 118 Kb CSU: Contacts of Structural Units for 1DI0 Likely Quarternary Molecular Structure file(s) for 1DI0 Retrieve 1DI0 in mmCIF format [Save to disk]

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1DI0, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1di0a_, region A [Jmol] [rasmolscript] [script source]     - Domain d1di0b_, region B [Jmol] [rasmolscript] [script source]     - Domain d1di0c_, region C [Jmol] [rasmolscript] [script source]     - Domain d1di0d_, region D [Jmol] [rasmolscript] [script source]     - Domain d1di0e_, region E [Jmol] [rasmolscript] [script source] Fold representative 1di0 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1di0_A] [1di0_E] [1di0_C] [1di0_B] [1di0_D]

Other resources with information on 1DI0

InterPro: IPR002180 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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