1FJ1 Immune System date Aug 07, 2000
title Lyme Disease Antigen Ospa In Complex With Neutralizing Antibody Fab La-2
authors W.Ding, C.L.Lawson
compound source
Molecule: Hybridoma Antibody La2 (Light Chain)
Chain: A, C
Fragment: Fab Fragment
Synonym: La2 Fab
Organism_scientific: Mus Musculus
Organism_common: Mouse
Other_details: La-2 Hybridoma, Gift Of M.M. Simon, Max Planck Instutut, Freiburg;

Molecule: Hybridoma Antibody La2 (Heavy Chain)
Chain: B, D
Fragment: Fab Fragment
Synonym: La2 Fab

Organism_scientific: Mus Musculus
Organism_common: Mouse
Other_details: La-2 Hybridoma

Molecule: Outer Surface Protein A
Chain: E, F
Synonym: Ospa
Engineered: Yes

Organism_scientific: Borrelia Burgdorferi
Organism_common: Lyme Disease Spirochete
Strain: B31
Expression_system: Escherichia Coli
Expression_system_plasmid: Pet9c
symmetry Space Group: P 21 21 2
R_factor 0.226
crystal
cell
length a length b length c angle alpha angle beta angle gamma
100.079 129.462 143.738 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.68 Å
note 1FJ1 supersedes 2OSP
related structures by homologous chain: 1CBV, 1CF8, 1FVE, 1RJL
similarity Belongs to the Lipoprotein_1 family.
subcellular loc. Attached to the outer membrane by a lipid anchor.
Gene BB ; OSPA (B. burgdorferi)
Gene
Ontology
ChainFunctionProcessComponent
F, E

  • cell outer membrane
  • membrane
  • outer membrane
  • Primary referenceStructural identification of a key protective B-cell epitope in Lyme disease antigen OspA., Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL, J Mol Biol 2000 Oct 6;302(5):1153-64. PMID:11183781
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (202 Kb) [Save to disk]
  • Biological Unit Coordinates (1fj1.pdb1.gz) 104 Kb
  • Biological Unit Coordinates (1fj1.pdb2.gz) 98 Kb
  • CSU: Contacts of Structural Units for 1FJ1
  • Likely Quarternary Molecular Structure file(s) for 1FJ1
  • Structure Factors (394 Kb)
  • Retrieve 1FJ1 in mmCIF format [Save to disk]
  • View 1FJ1 in 3D
  • Proteopedia, because life has more than 2D.
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
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  • Visual 3D analysis of 1FJ1
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  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electron Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1FJ1, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1fj1a1, region A:1-107 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1a2, region A:108-213 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1b1, region B:1-114 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1b2, region B:115-213 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1c1, region C:1-107 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1c2, region C:108-213 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1d1, region D:1-114 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1d2, region D:115-213 [Jmol] [rasmolscript] [script source]
        - Domain d1fj1e_, region E [Jmol] [rasmolscript] [script source]
        - Domain d1fj1f_, region F [Jmol] [rasmolscript] [script source]
  • Fold representative 1fj1 from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1fj1_D] [1fj1_C] [1fj1_B] [1fj1_A] [1fj1_E] [1fj1_F]
  • Other resources with information on 1FJ1
  • InterPro: IPR001809
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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