1G8Z Toxin date Nov 21, 2000
title His57ala Mutant Of Cholera Toxin B-Penatmer
authors A.T.Aman, S.Fraser, E.A.Merritt, C.Rodigherio, M.Kenny
compound source
Molecule: Cholera Toxin B Protein
Chain: D, E, F, G, H
Synonym: Ctb
Engineered: Yes
Mutation: Yes
Organism_scientific: Vibrio Cholerae
Organism_common: Bacteria
Gene: Ctxb
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
symmetry Space Group: P 21 21 2
R_factor 0.179
crystal
cell
length a length b length c angle alpha angle beta angle gamma
101.362 114.722 45.591 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.00 Å
ligand GAL enzyme
similarity Belongs to the Enterotoxin_b family.
subunit The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit.
gene ctxB (V. cholerae)
function It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself.
Gene
Ontology
ChainFunctionProcessComponent
H, F, D, G, E
  • pathogenesis
  • extracellular region
  • membrane
  • Primary referenceA mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A 2001 Jul 17;98(15):8536-41. PMID:11447291
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (105 Kb) [Save to disk]
  • Biological Unit Coordinates (1g8z.pdb1.gz) 92 Kb
  • CSU: Contacts of Structural Units for 1G8Z
  • Likely Quarternary Molecular Structure file(s) for 1G8Z
  • Structure Factors (521 Kb)
  • Retrieve 1G8Z in mmCIF format [Save to disk]
  • View 1G8Z in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1G8Z
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electron Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1G8Z, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1g8zd_, region D [Jmol] [rasmolscript] [script source]
        - Domain d1g8ze_, region E [Jmol] [rasmolscript] [script source]
        - Domain d1g8zf_, region F [Jmol] [rasmolscript] [script source]
        - Domain d1g8zg_, region G [Jmol] [rasmolscript] [script source]
        - Domain d1g8zh_, region H [Jmol] [rasmolscript] [script source]
  • Fold representative 1g8z from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1g8z_E] [1g8z_D] [1g8z_F] [1g8z_G] [1g8z_H]
  • Other resources with information on 1G8Z
  • InterPro: IPR001835
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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