1I4G Toxin date Feb 21, 2001
title Crystal Structure Of Staphylococcal Enterotoxin A Mutant H187a With Reduced Zn2+ Affinity
authors M.Hakansson, P.Antonsson, P.Bjork, L.A.Svensson
compound source
Molecule: Enterotoxin Type A
Chain: A, B
Synonym: Sea
Engineered: Yes
Mutation: Yes
Organism_scientific: Staphylococcus Aureus
Organism_common: Bacteria
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Ul635
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Pkp889
symmetry Space Group: P 21 21 21
R_factor 0.178
crystal
cell
length a length b length c angle alpha angle beta angle gamma
73.660 84.830 86.350 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.10 Å
ligand SO4, ZN enzyme
related structures by homologous chain: 1ESF, 1GXS, 1SXT
similarity Belongs to the staphylococcal/streptococcal toxin family.[Stap_Strp_tox_C]
subunit Monomer.
subcellular loc. Secreted protein.
Gene ENTA (S. aureus)
function The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome.
Gene
Ontology
ChainFunctionProcessComponent
A, B
  • zinc ion binding
  • metal ion binding
  • pathogenesis
  • extracellular region
  • Primary referenceCooperative zinc binding in a staphylococcal enterotoxin A mutant mimics the SEA-MHC class II interaction., Hakansson M, Antonsson P, Bjork P, Svensson LA, J Biol Inorg Chem 2001 Oct;6(8):757-62. PMID:11713682
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (83 Kb) [Save to disk]
  • Biological Unit Coordinates (1i4g.pdb1.gz) 43 Kb
  • Biological Unit Coordinates (1i4g.pdb2.gz) 39 Kb
  • CSU: Contacts of Structural Units for 1I4G
  • Likely Quarternary Molecular Structure file(s) for 1I4G
  • Retrieve 1I4G in mmCIF format [Save to disk]
  • View 1I4G in 3D
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  • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1I4G, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1i4ga1, region A:10-120 [Jmol] [rasmolscript] [script source]
        - Domain d1i4ga2, region A:121-233 [Jmol] [rasmolscript] [script source]
        - Domain d1i4gb2, region B:121-233 [Jmol] [rasmolscript] [script source]
        - Domain d1i4gb1, region B:8-120 [Jmol] [rasmolscript] [script source]
  • Fold representative 1i4g from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1i4g_B] [1i4g_A]
  • Other resources with information on 1I4G
  • InterPro: IPR006123 , IPR006173 , IPR006177 , IPR006126
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
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