1I4H Toxin date Feb 21, 2001
title Crystal Structure Of Zn2+ Soaked Staphylococcal Enterotoxin A Mutant H187a
authors M.Hakansson, P.Antonsson, P.Bjork, L.A.Svensson
compound source
Molecule: Enterotoxin Type A
Chain: A, B
Synonym: Sea
Engineered: Yes
Mutation: Yes
Organism_scientific: Staphylococcus Aureus
Organism_common: Bacteria
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Ul635
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Pkp889
symmetry Space Group: P 21 21 21
R_factor 0.208
crystal
cell
length a length b length c angle alpha angle beta angle gamma
74.110 85.060 86.540 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.90 Å
ligand ZN enzyme
related structures by homologous chain: 1SXT
similarity Belongs to the staphylococcal/streptococcal toxin family.[Stap_Strp_tox_C]
subunit Monomer.
subcellular loc. Secreted protein.
Gene ENTA (S. aureus)
function The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome.
Gene
Ontology
ChainFunctionProcessComponent
A, B
  • zinc ion binding
  • metal ion binding
  • pathogenesis
  • extracellular region
  • Primary referenceCooperative zinc binding in a staphylococcal enterotoxin A mutant mimics the SEA-MHC class II interaction., Hakansson M, Antonsson P, Bjork P, Svensson LA, J Biol Inorg Chem 2001 Oct;6(8):757-62. PMID:11713682
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (73 Kb) [Save to disk]
  • Biological Unit Coordinates (1i4h.pdb1.gz) 35 Kb
  • Biological Unit Coordinates (1i4h.pdb2.gz) 36 Kb
  • CSU: Contacts of Structural Units for 1I4H
  • Likely Quarternary Molecular Structure file(s) for 1I4H
  • Retrieve 1I4H in mmCIF format [Save to disk]
  • View 1I4H in 3D
  • Proteopedia, because life has more than 2D.
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1I4H
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1I4H, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1i4ha1, region A:10-120 [Jmol] [rasmolscript] [script source]
        - Domain d1i4ha2, region A:121-233 [Jmol] [rasmolscript] [script source]
        - Domain d1i4hb2, region B:121-233 [Jmol] [rasmolscript] [script source]
        - Domain d1i4hb1, region B:8-120 [Jmol] [rasmolscript] [script source]
  • Fold representative 1i4h from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1i4h_A] [1i4h_B]
  • Other resources with information on 1I4H
  • InterPro: IPR006123 , IPR006173 , IPR006177 , IPR006126
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

  • You may enter another PDB ID code
    Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]
    OCA© by Jaime Prilusky, 1996-2007
    Bioinformatics and Biological Computing
    Weizmann Institute of Science