1KZN	Isomerase	date	Feb 07, 2002
title	Crystal Structure Of E. Coli 24kda Domain In Complex With Clorobiocin
authors	D.Lafitte, V.Lamour, P.O.Tsvetkov, A.A.Makarov, M.Klich, P.Deprez, D.Moras, C.Briand, R.Gilli
compound		source
Molecule: Dna Gyrase Subunit B Chain: A Fragment: 24 Kda N-Terminus Domain Synonym: Gyrase B Ec: 5.99.1.3 Engineered: Yes		Organism_scientific: Escherichia Coli Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria
symmetry	Space Group: P 21 21 21	R_factor	0.239
crystal cell	length a length b length c angle alpha angle beta angle gamma 40.714 47.629 111.599 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.30 Å
ligand	CBN	enzyme	DNA topoisomerase (ATP-hydrolysing);. Type II DNA topoisomerase;. DNA-gyrase;. Deoxyribonucleate topoisomerase;. Deoxyribonucleic topoisomerase;. Topoisomerase;. DNA topoisomerase II. Isomerase E.C.5.99.1.3 BRENDA
similarity	Belongs to the type ii topoisomerase family.[DNA_gyraseB_C]
subunit	The enzyme forms an a2b2 tetramer. Made up of two chains. The a chain is responsible for dna breakage and rejoining; the b chain catalyzes atp hydrolysis.
catalytic activ.	Atp-dependent breakage, passage and rejoining of double-stranded dna.
enzyme regulation	Quinolones bind dna gyrase when the enzyme is complexed with dna and trap the enzyme in an abortive ternary complex. Target of two classes of inhibitors, coumarins and quinolones. Coumarins bind to gyrb and are competitive inhibitors with respect to atp.
genes	gyrA (B. burgdorferi); gyrA, nalA, parD (E. coli); top6A (M. jannaschii); top6B (S. shibatae); TOP2 (S. cerevisiae)
function	Dna gyrase negatively supercoils closed circular double- stranded dna in an atp-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded dna rings, including catenanes and knotted rings.
Primary reference	DNA gyrase interaction with coumarin-based inhibitors: the role of the hydroxybenzoate isopentenyl moiety and the 5'-methyl group of the noviose., Lafitte D, Lamour V, Tsvetkov PO, Makarov AA, Klich M, Deprez P, Moras D, Briand C, Gilli R, Biochemistry 2002 Jun 11;41(23):7217-23. PMID:12044152

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (45 Kb) [Save to disk] Biological Unit Coordinates (1kzn.pdb1.gz) 34 Kb CSU: Contacts of Structural Units for 1KZN Likely Quarternary Molecular Structure file(s) for 1KZN Structure Factors (126 Kb) Retrieve 1KZN in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1KZN, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1kzna_, region A [Jmol] [rasmolscript] [script source] Fold representative 1kzn from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1kzn_A]

Other resources with information on 1KZN

InterPro: IPR011557 , IPR011558 , IPR006171 , IPR000565 , IPR003594 , IPR001241 , IPR002288 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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