1LO5	Immune System Toxin	date	May 06, 2002
title	Crystal Structure Of The D227a Variant Of Staphylococcal Enterotoxin A In Complex With Human Mhc Class II
authors	K.Petersson, M.Thunnissen, G.Forsberg, B.Walse
compound		source
Molecule: Hla Class II Histocompatibility Antigen, Dr Alpha Chain; Chain: A Fragment: Extracellular Domain Synonym: Mhc Class II (Hla-Dr1, Dra 0101)-Chain A Engineered: Yes		Organism_scientific: Homo Sapiens Organism_common: Human Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_vector_type: Plasmid Expression_system_plasmid: Plm1
Molecule: Hla Class II Histocompatibility Antigen, Dr-1 Beta Chain; Chain: B Fragment: Extracellular Domain Synonym: Mhc Class II (Hla-Dr1, Drb1 0101)-Chain B Engineered: Yes		Organism_scientific: Homo Sapiens Organism_common: Human Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_vector_type: Plasmid Expression_system_plasmid: Plm1
Molecule: Hemagglutinin Peptide Chain: C Engineered: Yes		Synthetic: Yes Other_details: This Sequence Is Peptide From Influenza Virus, Hemagglutinin Peptide;
Molecule: Enterotoxin A Chain: D Engineered: Yes Mutation: Yes		Organism_scientific: Staphylococcus Aureus Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Ul635 Expression_system_vector_type: Plasmid Expression_system_plasmid: Plr16 Expression_system_gene: K12
symmetry	Space Group: P 21 21 21	R_factor	0.245
crystal cell	length a length b length c angle alpha angle beta angle gamma 52.008 75.792 198.082 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	3.20 Å
similarity	Belongs to the mhc class ii family. Belongs to the staphylococcal/streptococcal toxin family. Contains 1 ig-like c1-type (immunoglobulin-like) domain.[MHC_II_alpha]
subunit	Monomer. Heterodimer of an alpha chain and a beta chain.
polymorphism	The following alleles of drb1-1 are known: drb1*0101, drb1*0102, drb1*0103, drb1*0104, drb1*0105, drb1*0106 and drb1*0107. The sequence shown is that of dra*0101. The following alleles of dra are known dra*0102. The sequence shown is that of drb1*0101.
subcellular loc.	Secreted protein. Membrane; single-pass type i membrane protein.
Genes	ENTA (S. aureus); HLA-DRA1, HLA-DRA, HLA-DRB1 (H. sapiens)
function	The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome.
Gene Ontology	Chain Function Process Component A MHC class II receptor activi... antigen processing and prese... immune response antigen processing and prese... lysosome plasma membrane integral to plasma membrane membrane integral to membrane MHC class II protein complex... B antigen processing and prese... immune response antigen processing and prese... membrane integral to membrane MHC class II protein complex... D zinc ion binding metal ion binding pathogenesis extracellular region
Primary reference	Crystal structure of a SEA variant in complex with MHC class II reveals the ability of SEA to crosslink MHC molecules., Petersson K, Thunnissen M, Forsberg G, Walse B, Structure (Camb) 2002 Dec;10(12):1619-26. PMID:12467569

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (100 Kb) [Save to disk] Biological Unit Coordinates (1lo5.pdb1.gz) 96 Kb CSU: Contacts of Structural Units for 1LO5 Likely Quarternary Molecular Structure file(s) for 1LO5 Structure Factors (140 Kb) Retrieve 1LO5 in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1LO5, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1lo5a2, region A:4-81 [Jmol] [rasmolscript] [script source]     - Domain d1lo5a1, region A:82-182 [Jmol] [rasmolscript] [script source]     - Domain d1lo5b2, region B:3-92 [Jmol] [rasmolscript] [script source]     - Domain d1lo5b1, region B:93-190 [Jmol] [rasmolscript] [script source]     - Domain d1lo5d1, region D:1-120 [Jmol] [rasmolscript] [script source]     - Domain d1lo5d2, region D:121-233 [Jmol] [rasmolscript] [script source] Fold representative 1lo5 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1lo5_D] [1lo5_B] [1lo5_C] [1lo5_A]

Other resources with information on 1LO5

InterPro: IPR001003 , IPR007110 , IPR006173 , IPR003006 , IPR006177 , IPR000353 , IPR006126 , IPR006123 , IPR003597 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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