1M3J Toxin date Jun 28, 2002
title Crystal Form II Of Perfringolysin O
authors J.Rossjohn, M.Parker, G.Polekhina, S.Feil, R.Tweten
compound source
Molecule: Perfringolysin O
Chain: A, B
Engineered: Yes
 Organism_scientific: Clostridium Perfringens
Organism_common: Bacteria
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
symmetry Space Group: P 21 21 2
R_factor 0.247
crystal
cell
length a length b length c angle alpha angle beta angle gamma
167.000 214.110 47.070 90.00 90.00 90.00
method X-Ray Diffractionresolution 3.00 Å
related structures by homologous chain: 1M3I
similarity Belongs to the thiol-activated cytolysin family.[Thiol_cytolysin]
subunit Forms oligomers in the host membrane.
Genes CPE0163, PFOA, PFOR, PFO (C. perfringens)
function Cholesterol is the receptor for the binding of these toxins to eukaryotic cell membranes. Is able to lyse cholesterol containing membranes. Sulfhydryl-activated toxin. Can be reversibly inactivated by oxidation.
Gene
Ontology
ChainFunctionProcessComponent
A, B
  • lipid binding
  • cholesterol binding
  • pathogenesis
  • cytolysis
  • hemolysis by symbiont of hos...

    • Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (155 Kb) [Save to disk]
  • Biological Unit Coordinates (1m3j.pdb1.gz) 78 Kb
  • Biological Unit Coordinates (1m3j.pdb2.gz) 76 Kb
  • CSU: Contacts of Structural Units for 1M3J
  • Likely Quarternary Molecular Structure file(s) for 1M3J
  • Retrieve 1M3J in mmCIF format [Save to disk]
    • View 1M3J in 3D
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  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1M3J, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1m3ja_, region A [Jmol] [rasmolscript] [script source]
        - Domain d1m3jb_, region B [Jmol] [rasmolscript] [script source]
  • Fold representative 1m3j from FSSP and Dali (Families of Structurally Similar Proteins)
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  • Identification of Protein Pockets & Cavities at CASTp
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1m3j_A] [1m3j_B]
    • Other resources with information on 1M3J
  • InterPro: IPR001869
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