1OQV	Cell Adhesion	date	Mar 11, 2003
title	Structure Of Tcpa, The Type Iv Pilin Subunit From The Toxin Co-Regulated Pilus Of Vibrio Cholerae Classical Biotype
authors	L.Craig, J.A.Tainer
compound		source
Molecule: Toxin-Coregulated Pilus Subunit Chain: A, B, C Fragment: Globular Head Domain, Residues 29-199 Engineered: Yes		Organism_scientific: Vibrio Cholerae Organism_common: Bacteria Gene: Tcpa Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Origami Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet-15b
symmetry	Space Group: P 63	R_factor
crystal cell	length a length b length c angle alpha angle beta angle gamma 157.317 157.317 35.719 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	1.30 Å
ligand	GOL	enzyme
note	1OQV is a representative structure
domain	The leader sequence region and some other sequence particularities suggest that tcpa may represent a novel class of pilin, and imply the existence of a novel signal peptidase.
similarity	Belongs to the N_methyl family.
subcellular loc.	Secreted protein.
gene	tcpA (V. cholerae)
function	Constituent of pili, which may be involved in adhesion of v.cholerae to the host intestinal epithelium.
Gene Ontology	Chain Function Process Component A, C, B pathogenesis fimbrium extracellular organelle
Primary reference	Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin., Craig L, Taylor RK, Pique ME, Adair BD, Arvai AS, Singh M, Lloyd SJ, Shin DS, Getzoff ED, Yeager M, Forest KT, Tainer JA, Mol Cell 2003 May;11(5):1139-50. PMID:12769840

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (110 Kb) [Save to disk] Biological Unit Coordinates (1oqv.pdb1.gz) 43 Kb Biological Unit Coordinates (1oqv.pdb2.gz) 27 Kb Biological Unit Coordinates (1oqv.pdb3.gz) 27 Kb CSU: Contacts of Structural Units for 1OQV Likely Quarternary Molecular Structure file(s) for 1OQV Structure Factors (1827 Kb) Retrieve 1OQV in mmCIF format [Save to disk]

View 1OQV in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1OQV

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Structure-derived information

Conformational Epitope Prediction for 1OQV 1OQVA 1OQVB 1OQVC from the CEP Server. Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1OQV, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1oqva_, region A [Jmol] [rasmolscript] [script source]     - Domain d1oqvb_, region B [Jmol] [rasmolscript] [script source]     - Domain d1oqvc_, region C [Jmol] [rasmolscript] [script source] Fold representative 1oqv from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1oqv_A] [1oqv_B] [1oqv_C]

Other resources with information on 1OQV

InterPro: IPR010271 , IPR001120 , IPR012902 MMDB (Entrez's Structure Database)

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