1P4P Membrane Protein date Apr 23, 2003
title Outer Surface Protein B Of B. Burgdorferi: Crystal Structure Of The C-Terminal Fragment
authors M.Becker, J.Bunikis, B.D.Lade, J.J.Dunn, A.G.Barbour, C.L.Lawson
compound source
Molecule: Outer Surface Protein B
Chain: A
Fragment: Ospb C-Terminal Fragment
Synonym: Ospb
Engineered: Yes
Organism_scientific: Borrelia Burgdorferi
Organism_common: Lyme Disease Spirochete
Gene: Ospb
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Bl2(De3)Plyss
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Pet9c
symmetry Space Group: C 2 2 21
R_factor 0.211
crystal
cell
length a length b length c angle alpha angle beta angle gamma
30.800 51.660 158.820 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.00 Å
note 1P4P is a representative structure
related structures by homologous chain: 1RJL
similarity Belongs to the Lipoprotein_1 family.
subcellular loc. Attached to the outer membrane by a lipid anchor.
Gene BB ; OSPB (B. burgdorferi)
Gene
Ontology
ChainFunctionProcessComponent
A

  • cell outer membrane
  • membrane
  • outer membrane
  • Primary referenceStructural Investigation of Borrelia burgdorferi OspB, a BactericidalFab Target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (28 Kb) [Save to disk]
  • Biological Unit Coordinates (1p4p.pdb1.gz) 25 Kb
  • CSU: Contacts of Structural Units for 1P4P
  • Likely Quarternary Molecular Structure file(s) for 1P4P
  • Retrieve 1P4P in mmCIF format [Save to disk]
  • View 1P4P in 3D
  • Proteopedia, because life has more than 2D.
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1P4P
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1P4P, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1p4pa_, region A [Jmol] [rasmolscript] [script source]
  • Fold representative 1p4p from FSSP and Dali (Families of Structurally Similar Proteins)
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1p4p_A]
  • Other resources with information on 1P4P
  • InterPro: IPR001809
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

  • You may enter another PDB ID code
    Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]
    OCA© by Jaime Prilusky, 1996-2007
    Bioinformatics and Biological Computing
    Weizmann Institute of Science