1R8P	Transcription	date	Oct 28, 2003
title	Hpv-16 E2c Solution Structure
authors	A.D.Nadra, T.Eliseo, D.O.Cicero
compound		source
Molecule: Regulatory Protein E2 Chain: A, B Fragment: Dna Binding Domain Engineered: Yes		Organism_scientific: Human Papillomavirus Type 16 Organism_common: Virus Gene: E2 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Jm109 Expression_system_vector_type: Plasmid Expression_system_plasmid: Ptz18
method	NMR, 20 Structures
related structures	by homologous chain: 1A7G, 1DHM
similarity	Belongs to the papillomaviruses e2 protein family.[PPV_E2_C]
subunit	Binds dna as a dimer.
subcellular loc.	Nucleus.
Gene	E2 (H. papillomavirus)
function	The e1-e2 complex binds to the origin of dna replication. It can either activate or repress transcription depending on e2re's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. E2 regulates viral transcription and dna replication. It binds to the e2re response element (5'-accnnnnnnggt-3') present in multiple copies in the regulatory region.
Gene Ontology	Chain Function Process Component A, B nucleotide binding DNA binding transcription factor activit... DNA replication regulation of DNA replicatio... transcription regulation of transcription,... viral reproduction nucleus host cell nucleus
Primary reference	Solution structure of the HPV-16 E2 DNA binding domain, a transcriptional regulator with a dimeric beta-barrel fold., Nadra AD, Eliseo T, Mok YK, Almeida CL, Bycroft M, Paci M, de Prat-Gay G, Cicero DO, J Biomol NMR 2004 Oct;30(2):211-4. PMID:15702528

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (891 Kb) [Save to disk] Biological Unit Coordinates (1r8p.pdb1.gz) 889 Kb CSU: Contacts of Structural Units for 1R8P Original NMR restraints for 1R8P from PDB Experimental data, such as chemical shifts, at BioMagResBank (BMRB) NMR Restraints Grid from BioMagResBank (BMRB) Retrieve 1R8P in mmCIF format [Save to disk]

View 1R8P in 3D

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Visual 3D analysis of 1R8P

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1R8P, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1r8pa_, region A [Jmol] [rasmolscript] [script source]     - Domain d1r8pb_, region B [Jmol] [rasmolscript] [script source] Fold representative 1r8p from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1r8p_B] [1r8p_A]

Other resources with information on 1R8P

InterPro: IPR001866 , IPR012677 , IPR000427 NMR Ensemble (OLDERADO: core, domain and representative structure database) MMDB (Entrez's Structure Database)

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