1TS3	Toxin	date	Oct 10, 1997
title	H135a Mutant Of Toxic Shock Syndrome Toxin-1 From S. Aureus
authors	C.A.Earhart, D.T.Mitchell, D.L.Murray, D.M.Pinheiro, M.Matsumura, P.M.Schlievert, D.H.Ohlendorf
compound		source
Molecule: Toxic Shock Syndrome Toxin-1 Chain: A, B, C Synonym: Tsst-1 Engineered: Yes Mutation: Yes		Organism_scientific: Staphylococcus Aureus Expression_system: Staphylococcus Aureus
symmetry	Space Group: C 2 2 21	R_factor
crystal cell	length a length b length c angle alpha angle beta angle gamma 108.450 176.650 97.140 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.00 Å
related structures	by homologous chain: 1AW7, 2TSS
similarity	Belongs to the staphylococcal/streptococcal toxin family.[Stap_Strp_toxin]
subcellular loc.	Secreted protein.
Gene	TST (S. aureus)
function	Responsible for the symptoms of toxic shock syndrome.
Gene Ontology	Chain Function Process Component A, C, B pathogenesis extracellular region
Primary reference	Structures of five mutants of toxic shock syndrome toxin-1 with reduced biological activity., Earhart CA, Mitchell DT, Murray DL, Pinheiro DM, Matsumura M, Schlievert PM, Ohlendorf DH, Biochemistry 1998 May 19;37(20):7194-202. PMID:9585531

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (103 Kb) [Save to disk] Biological Unit Coordinates (1ts3.pdb1.gz) 38 Kb Biological Unit Coordinates (1ts3.pdb2.gz) 33 Kb Biological Unit Coordinates (1ts3.pdb3.gz) 33 Kb CSU: Contacts of Structural Units for 1TS3 Likely Quarternary Molecular Structure file(s) for 1TS3 Retrieve 1TS3 in mmCIF format [Save to disk]

View 1TS3 in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1TS3

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1TS3, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1ts3a1, region A:1-93 [Jmol] [rasmolscript] [script source]     - Domain d1ts3a2, region A:94-194 [Jmol] [rasmolscript] [script source]     - Domain d1ts3b1, region B:201-293 [Jmol] [rasmolscript] [script source]     - Domain d1ts3b2, region B:294-394 [Jmol] [rasmolscript] [script source]     - Domain d1ts3c1, region C:401-493 [Jmol] [rasmolscript] [script source]     - Domain d1ts3c2, region C:494-594 [Jmol] [rasmolscript] [script source] Fold representative 1ts3 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1ts3_B] [1ts3_A] [1ts3_C]

Other resources with information on 1TS3

InterPro: IPR006125 , IPR006123 , IPR006173 , IPR006126 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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