1VA5 Transferase date Feb 11, 2004
title Antigen 85c With Octylthioglucoside In Active Site
authors D.R.Ronning, V.Vissa, G.S.Besra, J.T.Belisle, J.C.Sacchettini, Tb Structural Genomics Consortium (Tbsgc)
compound source
Molecule: Antigen 85-C
Chain: A, B
Ec: 2.3.1.-
Engineered: Yes
Organism_scientific: Mycobacterium Tuberculosis
Organism_common: Bacteria
Gene: Fbpc2
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Bl21 (De3)
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Pet29
symmetry Space Group: C 1 2 1
R_factor 0.187
crystal
cell
length a length b length c angle alpha angle beta angle gamma
144.818 68.117 82.281 90.00 119.60 90.00
method X-Ray Diffractionresolution 2.02 Å
ligand SOG enzyme Transferase E.C.2.3.1 BRENDA
similarity Belongs to the mycobacterial a85 antigen family.[Esterase]
subcellular loc. Secreted.
function Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.
Gene
Ontology
ChainFunctionProcessComponent
A, B
  • acyltransferase activity
  • transferase activity


  • Primary referenceMycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity., Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC, J Biol Chem 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (112 Kb) [Save to disk]
  • Biological Unit Coordinates (1va5.pdb1.gz) 52 Kb
  • Biological Unit Coordinates (1va5.pdb2.gz) 46 Kb
  • CSU: Contacts of Structural Units for 1VA5
  • Likely Quarternary Molecular Structure file(s) for 1VA5
  • Structure Factors (374 Kb)
  • Retrieve 1VA5 in mmCIF format [Save to disk]
  • View 1VA5 in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1VA5
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electron Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1VA5, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1va5a_, region A [Jmol] [rasmolscript] [script source]
        - Domain d1va5b_, region B [Jmol] [rasmolscript] [script source]
  • Fold representative 1va5 from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1va5_A] [1va5_B]
  • Other resources with information on 1VA5
  • InterPro: IPR000801
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

  • You may enter another PDB ID code
    Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]
    OCA© by Jaime Prilusky, 1996-2006
    Bioinformatics and Biological Computing
    Weizmann Institute of Science