1XKP	Membrane Protein Chaperon	date	Sep 29, 2004
title	Crystal Structure Of The Virulence Factor Yopn In Complex With Its Heterodimeric Chaperone Sycn-Yscb
authors	F.D.Schubot, M.W.Jackson, K.J.Penrose, S.Cherry, J.E.Tropea, G.V.Plano, D.S.Waugh
compound		source
Molecule: Putative Membrane-Bound Yop Targeting Protein Yopn; Chain: A Fragment: Residues 32-277 Engineered: Yes Mutation: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Yopn Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pdest42
Molecule: Chaperone Protein Sycn Chain: B Engineered: Yes Mutation: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Sycn Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pdest42
Molecule: Chaperone Protein Yscb Chain: C Synonym: Yop Proteins Translocation Protein B Engineered: Yes Mutation: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Yscb Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pdest42
symmetry	Space Group: P 41	R_factor	0.198
crystal cell	length a length b length c angle alpha angle beta angle gamma 60.718 60.718 140.419 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	1.70 Å
ligand	DMG, MLY	enzyme
note	1XKP is a representative structure
similarity	Belongs to the hrpj/yopn family.[CesT]
subunit	Interacts with sycn to form a complex which specifically binds to yopn. Interacts with yscb to form a complex which specifically binds to yopn.
induction	Transcription is induced at 37 degrees celsius but down-regulated at this temperature by calcium.
subcellular loc.	Outer membrane (by similarity). Cytoplasmic or peripheral membrane protein that is not exported across the inner membrane.
Genes	SYCN, Y0030, Y0044, Y5027, Y5041, YPCD1.37C, YPCD1.51 (Y. pestis); YP (Y. pestis); YP (Y. pestis); YSCB (Y. pestis)
function	Seems to sense the calcium concentration and to transmit a signal to shut off yop transcription when the calcium concentration is high (by similarity). Functions as a specific chaperone for yopn. Plays a major role in regulation of the low-calcium response. It could facilitate the secretion and the subsequent translocation of yopn.
Gene Ontology	Chain Function Process Component B protein folding protein secretion cytoplasm membrane C protein folding pathogenesis regulation of protein secret... cytoplasm membrane
Primary reference	Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis., Schubot FD, Jackson MW, Penrose KJ, Cherry S, Tropea JE, Plano GV, Waugh DS, J Mol Biol 2005 Mar 4;346(4):1147-61. Epub 2005 Jan 20. PMID:15701523

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (88 Kb) [Save to disk] Biological Unit Coordinates (1xkp.pdb1.gz) 83 Kb CSU: Contacts of Structural Units for 1XKP Likely Quarternary Molecular Structure file(s) for 1XKP Structure Factors (410 Kb) Retrieve 1XKP in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1XKP, from MSDmotif at EBI Fold representative 1xkp from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1xkp_B] [1xkp_A] [1xkp_C]

Other resources with information on 1XKP

InterPro: IPR010261 , IPR012673 , IPR010812 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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