1Z7H	Hydrolase	date	Mar 24, 2005
title	2.3 Angstrom Crystal Structure Of Tetanus Neurotoxin Light Chain
authors	M.A.Breidenbach, A.T.Brunger
compound		source
Molecule: Tetanus Toxin Light Chain Chain: A Synonym: Tetanus Toxin Chain L Ec: 3.4.24.68 Engineered: Yes Mutation: Yes		Organism_scientific: Clostridium Tetani Organism_common: Bacteria Gene: Tetx Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21-De3 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet-28a
symmetry	Space Group: C 2 2 2	R_factor	0.212
crystal cell	length a length b length c angle alpha angle beta angle gamma 105.350 176.860 57.230 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.30 Å
ligand	ZN	enzyme	Tentoxilysin;. Tetanus neurotoxin. Hydrolase E.C.3.4.24.68 BRENDA
note	1Z7H is a representative structure
related structures	by homologous chain: 1YVG
similarity	Belongs to the peptidase m27 family.[Toxin_trans]
subunit	The precursor polypeptide is subsequently cleaved to yield subchains l and h. These remain linked by a disulfide bridge and are non-toxic after separation.
catalytic activ.	Hydrolysis of 76-gln-|-phe-77 bond in synaptobrevin 2.
Gene	CTC ; TETX (C. tetani)
function	It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. Tetanus toxin acts by inhibiting neurotransmitter release. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-gln-|-phe-77 bond of synaptobrevin-2.
Gene Ontology	Chain Function Process Component A metalloendopeptidase activit... peptidase activity metallopeptidase activity zinc ion binding hydrolase activity metal ion binding proteolysis pathogenesis extracellular region
Primary reference	2.3 a crystal structure of tetanus neurotoxin light chain(,)., Breidenbach MA, Brunger AT, Biochemistry 2005 May 24;44(20):7450-7. PMID:15895988

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (81 Kb) [Save to disk] Biological Unit Coordinates (1z7h.pdb1.gz) 77 Kb CSU: Contacts of Structural Units for 1Z7H Likely Quarternary Molecular Structure file(s) for 1Z7H Structure Factors (442 Kb) Retrieve 1Z7H in mmCIF format [Save to disk]

View 1Z7H in 3D

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1Z7H, from MSDmotif at EBI Fold representative 1z7h from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1z7h_A]

Other resources with information on 1Z7H

InterPro: IPR006025 , IPR012500 , IPR011591 , IPR012928 , IPR000395 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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