2BEO	Transcription	date	Nov 24, 2004
title	Prfa, Transcriptional Regulator In Listeria Monocytogenes
authors	M.Eiting, G.Hagelueken, W.D.Schubert, D.W.Heinz
compound		source
Molecule: Listeriolysin Regulatory Protein Chain: A, B Synonym: Prfa Engineered: Yes		Organism_scientific: Listeria Monocytogenes Strain: Egd-E Expression_system: Escherichia Coli Expression_system_strain: M15 Expression_system_plasmid: Pqe-30
symmetry	Space Group: P 1 21 1	R_factor	0.244
crystal cell	length a length b length c angle alpha angle beta angle gamma 55.951 80.713 60.965 90.00 111.91 90.00
method	X-Ray Diffraction	resolution	2.70 Å
ligand	ACM, CL, GLN, PG4	enzyme
related structures	by homologous chain: 1OMI, 2BGC
similarity	Contains1 hth crp-type dna-binding domain.
Genes	LMO0200, PRFA (L. monocytogenes)
function	Positively regulates expression of listeriolysin, of 1- phosphadidylinositol phosphodiesterase (pi-plc) and other virulence factors.
Primary reference	The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif., Eiting M, Hageluken G, Schubert WD, Heinz DW, Mol Microbiol. 2005 Apr;56(2):433-46. PMID:15813735

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (86 Kb) [Save to disk] Biological Unit Coordinates (2beo.pdb1.gz) 82 Kb CSU: Contacts of Structural Units for 2BEO Likely Quarternary Molecular Structure file(s) for 2BEO Structure Factors (93 Kb) Retrieve 2BEO in mmCIF format [Save to disk]

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2BEO, from MSDmotif at EBI Fold representative 2beo from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2beo_A] [2beo_B]

Other resources with information on 2BEO

InterPro: IPR012318 , IPR000595 , IPR011991 , IPR001808 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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