2FK4 Metal Binding Protein, Oncoprotein date Jan 04, 2006
title Solution Structure Of The C-Terminal Zinc Binding Domain Of The Hpv16 E6 Oncoprotein
authors Y.Nomine, S.Charbonnier
compound source
Molecule: Protein E6
Chain: A
Fragment: C-Terminal Domain
Engineered: Yes
Mutation: Yes
Organism_scientific: Human Papillomavirus Type 16
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Bl21 De2
Expression_system_vector_type: Plasmid
methodNMR, 10 Structures
ligand ZN enzyme
note 2FK4 is a representative structure
similarity Belongs to the papillomaviruses E6 protein family.
subunit Forms a complex with e6-ap ubiquitin-protein ligase which interacts with human p53. Binds to human fbln1 and mpdz (by similarity).
subcellular loc. Nuclear matrix-associated.
Gene E6 (H. papillomavirus)
function Interaction with human fbln1 protein also seems to be linked to cell transformation. Those two functions presumably contribute to transforming activity (by similarity). Transcriptional transactivator. Has transforming activity. Inactivates, with e6-ap ubiquitin- protein ligase, the human tp53/p53 tumor suppressor protein by targeting it to degradation. Binds and targets human mupp1/mpdz protein to degradation. Binds double stranded dna.
Gene
Ontology
ChainFunctionProcessComponent
A
  • DNA binding
  • protein binding
  • zinc ion binding
  • metal ion binding
  • transcription
  • regulation of transcription,...
  • nucleus
  • host cell nucleus
  • Primary referenceStructural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:16507364
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (213 Kb) [Save to disk]
  • Biological Unit Coordinates (2fk4.pdb1.gz) 211 Kb
  • CSU: Contacts of Structural Units for 2FK4
  • Original NMR restraints for 2FK4 from PDB
  • Retrieve 2FK4 in mmCIF format [Save to disk]
  • View 2FK4 in 3D
  • Proteopedia, because life has more than 2D.
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  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 2FK4, from MSDmotif at EBI
  • Fold representative 2fk4 from FSSP and Dali (Families of Structurally Similar Proteins)
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [2fk4_A]
  • Other resources with information on 2FK4
  • InterPro: IPR001334
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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