2ZNL	Transcription	date	Apr 28, 2008
title	Crystal Structure Of Pa-Pb1 Complex Form Influenza Virus Rna Polymerase
authors	E.Obayashi, H.Yoshida, F.Kawai, N.Shibayama, A.Kawaguchi, K.Nagata, J.R.H.Tame, S.Y.Park
compound		source
Molecule: Polymerase Acidic Protein Chain: A Fragment: Residues 239-716 Synonym: Rna-Directed Rna Polymerase Subunit P2 Engineered: Yes		Organism_scientific: Influenza A Virus Organism_taxid: 211044 Strain: Strain Apuerto Rico81934 H1n1 Gene: Pa Expression_system: Escherichia Coli Expression_system_taxid: 562 Expression_system_strain: Bl21(De3)Rilp Codonplus Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet28b
Molecule: Rna-Directed Rna Polymerase Catalytic Subunit Chain: B Fragment: Residues 1-81 Synonym: Polymerase Basic Protein 1, Pb1, Rna-Directed Rna Polymerase Subunit P1; Ec: 2.7.7.48 Engineered: Yes		Organism_scientific: Influenza A Virus Organism_taxid: 211044 Strain: Strain Apuerto Rico81934 H1n1 Gene: Pb1 Expression_system: Escherichia Coli Expression_system_taxid: 562 Expression_system_strain: Bl21(De3)Rilp Codonplus Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet28b
symmetry	Space Group: P 32 2 1	R_factor 0.207 R_Free 0.262
crystal cell	length a length b length c angle alpha angle beta angle gamma 101.957 101.957 115.023 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2 Å
ligand		enzyme	Transferase E.C.2.7.7.48 BRENDA
Genes	PA, PB1 (I. A)
Gene Ontology	Chain Function Process Component A RNA binding RNA-directed RNA polymerase ... transcription viral genome maturation nucleoplasm cytosol B nucleotide binding RNA-directed RNA polymerase ... transferase activity nucleotidyltransferase activ... transcription transcription, RNA-dependent... viral genome maturation viral genome replication nucleus nucleoplasm cytosol host cell nucleus
Primary reference	The structural basis for an essential subunit interaction in influenza virus RNA polymerase., Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR, Park SY, Nature. 2008 Aug 28;454(7208):1127-31. Epub 2008 Jul 27. PMID:18660801

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (79 Kb) [Save to disk] Biological Unit Coordinates (2znl.pdb1.gz) 73 Kb CSU: Contacts of Structural Units for 2ZNL Likely Quarternary Molecular Structure file(s) for 2ZNL Structure Factors (449 Kb) Retrieve 2ZNL in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2ZNL, from MSDmotif at EBI Fold representative 2znl from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2znl_B] [2znl_A]

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