ID   OS25_PLAFO              Reviewed;         217 AA.
AC   P13829;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   13-NOV-2007, entry version 52.
DE   25 kDa ookinete surface antigen precursor (Pfs25).
OS   Plasmodium falciparum (isolate NF54).
OC   Eukaryota; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodium; Plasmodium (Laverania).
OX   NCBI_TaxID=5843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=88202105; PubMed=3283563; DOI=10.1038/333074a0;
RA   Kaslow D.C., Quakyi I.A., Syin C., Raum M.G., Keister D.B.,
RA   Coligan J.E., McCutchan T.F., Miller L.H.;
RT   "A vaccine candidate from the sexual stage of human malaria that
RT   contains EGF-like domains.";
RL   Nature 333:74-76(1988).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor
CC       (Potential).
CC   -!- DEVELOPMENTAL STAGE: Expressed on zygotes and ookinetes.
CC   -!- SIMILARITY: Contains 4 EGF-like domains.
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DR   EMBL; X07802; CAA30646.1; -; Genomic_DNA.
DR   PIR; S00769; S00769.
DR   PDB; 2ICL; Model; -; @=23-195.
DR   InterPro; IPR006210; EGF.
DR   InterPro; IPR013032; EGF_like_reg.
DR   InterPro; IPR010423; Plasmod_Pvs28.
DR   Pfam; PF06247; Plasmod_Pvs28; 1.
DR   SMART; SM00181; EGF; 3.
DR   PROSITE; PS00022; EGF_1; FALSE_NEG.
DR   PROSITE; PS01186; EGF_2; 2.
DR   PROSITE; PS50026; EGF_3; FALSE_NEG.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; EGF-like domain;
KW   Glycoprotein; GPI-anchor; Lipoprotein; Malaria; Membrane; Repeat;
KW   Signal.
FT   SIGNAL        1     16
FT   CHAIN        17    196       25 kDa ookinete surface antigen.
FT                                /FTId=PRO_0000024567.
FT   PROPEP      197    217       Removed in mature form (Potential).
FT                                /FTId=PRO_0000024568.
FT   DOMAIN       30     59       EGF-like 1; truncated.
FT   DOMAIN       61    106       EGF-like 2.
FT   DOMAIN      106    150       EGF-like 3.
FT   DOMAIN      153    193       EGF-like 4.
FT   LIPID       196    196       GPI-anchor amidated serine (Potential).
FT   CARBOHYD    112    112       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    165    165       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    187    187       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    202    202       N-linked (GlcNAc...) (Potential).
FT   DISULFID     65     80       By similarity.
FT   DISULFID     74     92       By similarity.
FT   DISULFID     94    105       By similarity.
FT   DISULFID    110    120       By similarity.
FT   DISULFID    115    133       By similarity.
FT   DISULFID    135    149       By similarity.
FT   DISULFID    157    168       By similarity.
FT   DISULFID    161    177       By similarity.
FT   DISULFID    179    192       By similarity.
SQ   SEQUENCE   217 AA;  24143 MW;  19DC1F4CED7CF19F CRC64;
     MNKLYSLFLF LFIQLSIKYN NAKVTVDTVC KRGFLIQMSG HLECKCENDL VLVNEETCEE
     KVLKCDEKTV NKPCGDFSKC IKIDGNPVSY ACKCNLGYDM VNNVCIPNEC KNVTCGNGKC
     ILDTSNPVKT GVCSCNIGKV PNVQDQNKCS KDGETKCSLK CLKENETCKA VDGIYKCDCK
     DGFIIDNESS ICTAFSAYNI LNLSIMFILF SVCFFIM
//