1HRK	Lyase	date	Dec 21, 2000
title	Crystal Structure Of Human Ferrochelatase
authors	C.K.Wu, H.A.Dailey, J.P.Rose, A.Burden, V.M.Sellers, B.-C.Wang
compound		source
Molecule: Ferrochelatase Chain: A, B Fragment: Mature Length Synonym: Protoheme Ferro-Lyase, Heme Synthetase Ec: 4.99.1.1 Engineered: Yes Mutation: Yes		Organism_scientific: Homo Sapiens Organism_common: Human Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Jm109 Expression_system_vector_type: Plasmid Expression_system_plasmid: Phdtf20
symmetry	Space Group: P 21 21 21	R_factor	0.202
crystal cell	length a length b length c angle alpha angle beta angle gamma 93.433 87.569 109.699 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.00 Å
ligand	CHD, FES	enzyme	Ferrochelatase. Ferro-protoporphyrin chelatase. Iron chelatase. Heme synthetase. Heme synthase. Lyase E.C.4.99.1.1
note	1HRK is a representative structure
similarity	Belongs to the Ferrochelatase family.
subunit	Homodimer.
catalytic activ.	Protoporphyrin + fe(2+) = protoheme + 2 h(+).
pathway	Protoheme biosynthesis; last step.
subcellular loc.	Bound to the mitochondrial inner membrane in eukaryotic cells with its active site on the matrix side of the membrane.
enzyme regulation	The 2fe-2s cluster could act as a no sensor. Inhibited by nitric oxide (no).
genes	BSU10130, HEMF, HEMH (B. subtilis); FECH (H. sapiens); HEM15, MET8, YBR1461, YBR213W, YOR176W (S. cerevisiae)
function	Catalyzes the ferrous insertion into protoporphyrin ix.
Gene Ontology	chain A: GO:0004325, GO:0005739, GO:0006091, GO:0006779, GO:0006783, GO:0008198, GO:0009416, GO:0016829 chain B: GO:0004325, GO:0005739, GO:0006091, GO:0006779, GO:0006783, GO:0008198, GO:0009416, GO:0016829
disease	Protoporphyria,erythropoietic Protoporphyria,erythropoietic,recessive,with liver failure Depending on the mutation, it can sometimes be recessive. Epp is an dominantly inherited disease of porphyrin metabolism. Defects in fech are the cause of erythropoietic protoporphyria (epp) [mim. 177000]. The clinical manifestations are photosensitivity and hepatobiliary disease.
Primary reference	The 2.0 A structure of human ferrochelatase, the terminal enzyme of heme biosynthesis., Wu CK, Dailey HA, Rose JP, Burden A, Sellers VM, Wang BC, Nat Struct Biol 2001 Feb;8(2):156-60. PMID:11175906

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (147 Kb) [Save to disk] Biological Unit Coordinates (1hrk.pdb1.gz) 130 Kb LPC: Ligand-Protein Contacts for 1HRK CSU: Contacts of Structural Units for 1HRK Likely Quarternary Molecular Structure file(s) for 1HRK Structure Factors (r1hrksf.ent.Z) 497 Kb Retrieve 1HRK in mmCIF format [Save to disk]

View 1HRK in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1HRK

STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure. Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges. GRASS (Graphical Representation and Analysis of Structure Server) Cartoon representation from PDB Cartoon Ramachandran plot from PDBSum

Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1hrka_, region A [Jmol] [rasmolscript] [script source]     - Domain d1hrkb_, region B [Jmol] [rasmolscript] [script source] Fold representative 1hrk from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH Summaries and structural analyses of PDB data files: PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1hrk_A] [1hrk_B] SWISS-PROT database: [P22830] Domain organization of [HEMZ_HUMAN] by SWISSPFAM Conserved protein region description, domain view and alignment from family Ferrochelatase (PF00762) of Pfam. Alignments of the sequence of 1HRK with the sequences similar proteins can be viewed for classification [HEMZ_HUMAN] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list. A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [HEMZ_HUMAN] at ProtoMap. Click on the Cluster number.

Other resources with information on 1HRK

InterPro: IPR001015 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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