1HZJ Isomerase date Jan 25, 2001
title Human Udp-Galactose 4-Epimerase: Accommodation Of Udp-N- Acetylglucosamine Within The Active Site
authors J.B.Thoden, T.M.Wohlers, J.L.Fridovich-Keil, H.M.Holden
compound source
Molecule: Udp-Galactose 4-Epimerase
Chain: A, B
Synonym: Udp-Glucose 4-Epimerase
Ec: 5.1.3.2
Engineered: Yes
 Organism_scientific: Homo Sapiens
Organism_common: Human
Expression_system: Pichia Pastoris
Expression_system_common: Fungi
Expression_system_strain: Gs115
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Ppic3.5khgale
symmetry Space Group: P 21 21 21
R_factor 0.184
crystal
cell
length a length b length c angle alpha angle beta angle gamma
63.400 88.900 118.700 90.00 90.00 90.00
method X-Ray Diffractionresolution 1.50 Å
ligand CL, MG, NAD, UD1enzyme UDP-glucose 4-epimerase. UDP-galactose 4-epimerase. Uridine diphosphoglucose epimerase. Galactowaldenase. UDPG-4-epimerase. Uridine diphosphate galactose 4-epimerase. Uridine diphospho-galactose-4-epimerase. UDP-glucose epimerase. 4-epimerase. Uridine diphosphoglucose 4-epimerase. Uridine diphosphate glucose 4-epimerase. UDP-D-galactose 4-epimerase. Isomerase E.C.5.1.3.2
related structures by homologous chain: 1I3L, 1I3M
similarity Belongs to the sugar Epimerase family.
catalytic activ. Udp-glucose = udp-galactose.
pathway Galactose metabolism; third step.
genes B0759, GALD, GALE (E. coli); GALE (H. sapiens)
function Catalyzes two distinct but analogous reactions epimerization of udp-glucose to udp-galactose and the epimerization of udp-n-acetylglucosamine to udp-n- acetylgalactosamine.
Gene
Ontology 		chain A: GO:0003824, GO:0003978, GO:0005975, GO:0006012, GO:0009225, GO:0016853, GO:0016857
chain B: GO:0003824, GO:0003978, GO:0005975, GO:0006012, GO:0009225, GO:0016853, GO:0016857
disease Galactose epimerase deficiency
There are two clinically distinct forms of edg. Defects in gale are the cause of epimerase-deficiency galactosemia (edg) [mim. 230350]; also known as galactosemia iii:(1) a benign, or 'peripheral' form with no detectable gale activity in red blood cells:(2) a much rarer and 'generalized' form with undetectable levels of gale activity in all tissues examined.
Primary referenceHuman UDP-galactose 4-epimerase. Accommodation of UDP-N-acetylglucosamine within the active site., Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM, J Biol Chem 2001 May 4;276(18):15131-6. PMID:11279032
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (146 Kb) [Save to disk]
  • Biological Unit Coordinates (1hzj.pdb1.gz) 132 Kb
  • LPC: Ligand-Protein Contacts for 1HZJ
  • CSU: Contacts of Structural Units for 1HZJ
  • Likely Quarternary Molecular Structure file(s) for 1HZJ
  • Structure Factors (r1hzjsf.ent.Z) 982 Kb
  • Retrieve 1HZJ in mmCIF format [Save to disk]
    • View 1HZJ in 3D
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  • Known point mutations with 6 PDB ids, from MutaProt at Weizmann Institute
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  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1hzja_, region A [Jmol] [rasmolscript] [script source]
        - Domain d1hzjb_, region B [Jmol] [rasmolscript] [script source]
  • Fold representative 1hzj from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1hzj_A] [1hzj_B]
  • SWISS-PROT database: [Q14376] [Q9BVE3] [Q9UJB4] [Q86W41]
  • Domain organization of [GALE_HUMAN] by SWISSPFAM
  • Conserved protein region description, domain view and alignment from family Epimerase (PF01370) of Pfam.
  • Alignments of the sequence of 1HZJ with the sequences similar proteins can be viewed for classification [GALE_HUMAN] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [GALE_HUMAN] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 1HZJ
  • InterPro: IPR008089 , IPR005886 , IPR001509
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