1IAT Isomerase date Mar 23, 2001
title Crystal Structure Of Human Phosphoglucose Isomeraseneuroleukinautocrine Motility Factormaturation Factor
authors J.A.Read, J.Pearce, X.Li, H.Muirhead, J.Chirgwin, C.Davies
compound source
Molecule: Phosphoglucose Isomerase
Chain: A
Synonym: Glucose-6-Phosphate Isomerase, Glucose Phosphate Isomerase;
Ec: 5.3.1.9
Engineered: Yes
Organism_scientific: Homo Sapiens
Organism_common: Human
Gene: Gpi
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
symmetry Space Group: P 43 21 2
R_factor 0.144
crystal
cell
length a length b length c angle alpha angle beta angle gamma
94.183 94.183 136.138 90.00 90.00 90.00
method X-Ray Diffractionresolution 1.62 Å
ligand SUL, BMEenzyme Glucose-6-phosphate isomerase. Phosphohexose isomerase. Phosphohexomutase. Oxoisomerase. Hexosephosphate isomerase. Phosphosaccharomutase. Phosphoglucoisomerase. Phosphohexoisomerase. Phosphoglucose isomerase. Glucose phosphate isomerase. Hexose phosphate. Isomerase E.C.5.3.1.9
note 1IAT is a representative structure
related structures by homologous chain: 1HM5
similarity Belongs to the gpi family.[PGI]
subunit Homodimer.
catalytic activ. D-glucose 6-phosphate = d-fructose 6- phosphate.
pathway Involved in glycolysis and in gluconeogenesis.
subcellular loc. Cytoplasmic.
genes PGIB (B. stearothermophilus); GPI (H. sapiens); GPI (S. scrofa); PF0196, PGIA (P. furiosus); GPI (O. cuniculus)
function Neuroleukin is a neurotrophic factor for spinal and sensory neurons.
Gene
Ontology
chain A: GO:0004347, GO:0005125, GO:0005975, GO:0006094, GO:0006096, GO:0006959, GO:0007399, GO:0007599, GO:0008083, GO:0016853
disease Hemolytic anemia due to glucosephosphate isomerase deficiency
Hydrops fetalis,one form
Defects in gpi are a cause of hereditary nonspherocytic hemolytic anemia (ha) [mim. 172400]. Severe gpi deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Primary referenceThe crystal structure of human phosphoglucose isomerase at 1.6 A resolution: implications for catalytic mechanism, cytokine activity and haemolytic anaemia., Read J, Pearce J, Li X, Muirhead H, Chirgwin J, Davies C, J Mol Biol 2001 Jun 1;309(2):447-63. PMID:11371164
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (224 Kb) [Save to disk]
  • Biological Unit Coordinates (1iat.pdb1.gz) 396 Kb
  • LPC: Ligand-Protein Contacts for 1IAT
  • CSU: Contacts of Structural Units for 1IAT
  • Likely Quarternary Molecular Structure file(s) for 1IAT
  • Structure Factors (r1iatsf.ent.Z) 716 Kb
  • Retrieve 1IAT in mmCIF format [Save to disk]
  • View 1IAT in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
  • Visual 3D analysis of 1IAT
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electro Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1iata_, region A [Jmol] [rasmolscript] [script source]
  • Fold representative 1iat from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1iat_A]
  • SWISS-PROT database: [Q9UHE6] [P06744] [Q9BSK5]
  • Domain organization of [G6PI_HUMAN] by SWISSPFAM
  • Conserved protein region description, domain view and alignment from family PGI (PF00342) of Pfam.
  • Alignments of the sequence of 1IAT with the sequences similar proteins can be viewed for classification [G6PI_HUMAN] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [G6PI_HUMAN] at ProtoMap. Click on the Cluster number.
  • Other resources with information on 1IAT
  • InterPro: IPR001672
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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