1SBW Hydrolase Hydrolase Inhibitor date Apr 29, 1999
title Crystal Structure Of Mung Bean Inhibitor Lysine Active Fragment Complex With Bovine Beta-Trypsin At 1.8a Resolutio
authors Y.Zhu, Q.Huang, C.Chi
compound source
Molecule: Beta-Trypsin
Chain: A
Ec: 3.4.21.4
 Organism_scientific: Bos Taurus
Organism_common: Bovine
Organ: Pancreatic

Molecule: Mung Bean Inhibitor Lysin Active Frament
Chain: I
Organism_scientific: Vigna Radiata
Organism_common: Mung Bean
symmetry Space Group: P 21 21 21
R_factor 0.164
crystal
cell
length a length b length c angle alpha angle beta angle gamma
63.270 63.550 69.440 90.00 90.00 90.00
method X-Ray Diffractionresolution 1.80 Å
ligand CA, SO4enzyme Trypsin. Alpha-trypsin. Beta-trypsin. Cocoonase. Parenzyme. Parenzymol. Tryptar. Trypure. Pseudotrypsin. Tryptase. Tripcellim. Sperm receptor hydrolase. Hydrolase E.C.3.4.21.4
related structures by homologous chain: 1BTW, 1TNH
similarity Belongs to the bowman-birk serine protease inhibitor family. Belongs to peptidase family s1.[trypsin]
catalytic activ. Preferential cleavage.
post-translat. modifications Further cleavage after lys-190 yields pseudotrypsin. Subsequent cleavage after lys-145 leads to alpha-trypsin. A cleavage may also occur after arg-119. Autocatalytic cleavage after lys-20 leads to beta-trypsin by releasing a terminal hexapeptide.
tissue Synthesized in the acinar cells of the pancreas.
subcellular loc. Extracellular.
genes B2209, ECO, ETI (E. coli); A4, AAT, AD1, APP, LACI, PI, PRSS1, PRSS3, PRSS4, SERPINA1, TFPI, TFPI1, TRP1, TRY1, TRY3, TRY4, TRYP1 (H. sapiens); TRY2 (R. norvegicus); KTI3 (G. max); SSI (S. albogriseolus); SPRT (S. griseus)
Gene
Ontology 		chain A: GO:0004252, GO:0004263, GO:0004295, GO:0005509, GO:0006508, GO:0007586, GO:0016787
chain I: GO:0004867, GO:0005576
disease Emphysema
Emphysema-cirrhosis
Hemorrhagic diathesis due to\`antithrombin\' Pittsburgh
Alzheimer disease-1,APP-related
Amyloidosis,cerebroarterial,Dutch type
Schizophrenia,chronic
Pancreatitis,hereditary
Trypsinogen deficiency
Primary referenceCrystal structure of mung bean inhibitor lysine active fragment complex with bovine beta-trypsin at 1.8A resolution., Zhu Y, Huang Q, Chi C, J Biomol Struct Dyn 1999 Jun;16(6):1219-24. PMID:10447205
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (53 Kb) [Save to disk]
  • Biological Unit Coordinates (1sbw.pdb1.gz) 42 Kb
  • LPC: Ligand-Protein Contacts for 1SBW
  • CSU: Contacts of Structural Units for 1SBW
  • Likely Quarternary Molecular Structure file(s) for 1SBW
  • Structure Factors (r1sbwsf.ent.Z) 171 Kb
  • Retrieve 1SBW in mmCIF format [Save to disk]
    • View 1SBW in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
    • Visual 3D analysis of 1SBW
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
    • Structure-derived information
  • Electro Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Known point mutation with 1 PDB id, from MutaProt at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1sbwa_, region A [Jmol] [rasmolscript] [script source]
  • Fold representative 1sbw from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1sbw_I] [1sbw_A]
  • SWISS-PROT database: [P00760] [P01062]
  • Domain organization of [TRY1_BOVIN] [IBB_PHAAU] by SWISSPFAM
  • Domains found in 1SBW: [BowB] [Tryp_SPc ] by SMART
  • Conserved protein region description, domain view and alignment from family trypsin (PF00089) of Pfam.
  • Alignments of the sequence of 1SBW with the sequences similar proteins can be viewed for classification [TRY1_BOVIN] [IBB_PHAAU] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [TRY1_BOVIN] [IBB_PHAAU] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 1SBW
  • InterPro: IPR001314 , IPR000877 , IPR009003 , IPR001254
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

    • You may enter another PDB ID code
    Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]
    OCA© by Jaime Prilusky, 1996-2004
    Bioinformatics and Biological Computing
    Weizmann Institute of Science