1TAU Complex (Nucleotidyltransferase Dna) date Jun 17, 1996
title Structure Of Dna Polymerase
authors S.H.Eom, J.Wang, T.A.Steitz
compound source
Molecule: Taq Dna Polymerase
Chain: A
Synonym: Taq
Ec: 2.7.7.7
Engineered: Yes
 Organism_scientific: Thermus Aquaticus
Expression_system: Escherichia Coli

Molecule: Dna
Chain: T, P
Engineered: Yes
Synthetic: Yes
symmetry Space Group: P 31 2 1
R_factor 0.244
crystal
cell
length a length b length c angle alpha angle beta angle gamma
107.600 107.600 170.010 90.00 90.00 120.00
method X-Ray Diffraction, Single Crystalresolution 3.0 Å
ligand BGL, ZNenzyme DNA-directed DNA polymerase. DNA polymerase I. DNA polymerase II. DNA polymerase III. DNA polymerase alpha. DNA polymerase beta. DNA polymerase gamma. DNA nucleotidyltransferase (DNA-directed). Deoxyribonucleate nucleotidyltransferase. Deoxynucleate polymerase. Deoxyribonucleic acid duplicase. Deoxyribonucleic acid polymerase. Deoxyribonucleic duplicase. Deoxyribonucleic polymerase. Deoxyribonucleic polymerase I. DNA duplicase. DNA nucleotidyltransferase. DNA polymerase. DNA replicase. DNA-dependent DNA polymerase. Duplicase. Klenow fragment. Sequenase. Taq DNA polymerase. Taq Pol I. Tca DNA polymerase. Transferase E.C.2.7.7.7
related structures by homologous chain: 1JXE
similarity Belongs to the dna polymerase type-a family.[5_3_exonuclease]
catalytic activ. N deoxynucleoside triphosphate = n diphosphate + {dna}(n).
genes POL, POLA (B. stearothermophilus); 43 (B. RB69); 43 (B. T4); 5 (B. T7); BALF5 (E. virus); B0060, B0215, B0470, B0640, B1099, B1842, B3701, B3863, C2252, C4623, DINA, DNAN, DNAQ, DNAX, DNAZ, DNAZX, ECS2552, ECS4636, HOLA, HOLB, HOLE, MUTD, OR, POLA, POLB, RESA, S1918, S3905, SF1852.1, SF3854, STMD1.75, STY3639, T3381, Z2891, Z5192 (E. coli); UL42 (H. herpesvirus); UL30 (H. herpesvirus); HLA-DRA, POLA, POLB (H. sapiens); POL (M. murine); POL (P. kodakaraensis); POLB (R. norvegicus); DBH, DPO4, SSO2448 (S. solfataricus); POL1, POLA (T. aquaticus); POL, POLA (T. gorgonarius); POL, POLA (T. sp.)
Gene
Ontology 		chain A: GO:0003677, GO:0003887, GO:0004518, GO:0005622, GO:0006260, GO:0006281, GO:0008409, GO:0016740
disease N syndrome
Primary referenceStructure of Taq ploymerase with DNA at the polymerase active site., Eom SH, Wang J, Steitz TA, Nature 1996 Jul 18;382(6588):278-81. PMID:8717047
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (182 Kb) [Save to disk]
  • Biological Unit Coordinates (1tau.pdb1.gz) 135 Kb
  • LPC: Ligand-Protein Contacts for 1TAU
  • CSU: Contacts of Structural Units for 1TAU
  • Likely Quarternary Molecular Structure file(s) for 1TAU
  • Structure Factors (r1tausf.ent.Z) 206 Kb
  • Retrieve 1TAU in mmCIF format [Save to disk]
    • View 1TAU in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On AstexViewer, from MSD-EBI (viewer documentation).
  • On RasMol (Install RasMol freeware) Here's help on how to use RasMol.
    • Visual 3D analysis of 1TAU
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
    • View 1TAU in 2D
  • Topology of domains/folds: Chain: A from TOPS (The Atlas of protein topology cartoons )
    • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Known point mutations with 2 PDB ids, from MutaProt at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • Genome occurence of 1TAU's fold from GeneCensus
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1taua2, region A:10-173 [Jmol] [rasmolscript] [script source]
        - Domain d1taua1, region A:174-289 [Jmol] [rasmolscript] [script source]
        - Domain d1taua3, region A:290-422 [Jmol] [rasmolscript] [script source]
        - Domain d1taua4, region A:423-832 [Jmol] [rasmolscript] [script source]
  • Fold representative 1tau from FSSP and Dali (Families of Structurally Similar Proteins)
  • Domain Definition for 1tau from 3Dee ( The Database of Protein Domain Definitions )
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1tau_P] [1tau_A] [1tau_T]
  • SWISS-PROT database: [P19821]
  • Domain organization of [DPO1_THEAQ] by SWISSPFAM
  • Domains found in 1TAU: [53EXOc] [HhH1] [HhH2] [POLAc ] by SMART
  • Conserved protein region description, domain view and alignment from family 5_3_exonuclease (PF01367) of Pfam.
  • Alignments of the sequence of 1TAU with the sequences similar proteins can be viewed for classification [DPO1_THEAQ] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [DPO1_THEAQ] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 1TAU
  • InterPro: IPR002421 , IPR002298 , IPR000513 , IPR003583 , IPR001098 , IPR008918
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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