2SRC Tyrosine-Protein Kinase date Dec 29, 1998
title Crystal Structure Of Human Tyrosine-Protein Kinase C-Src, In Complex With Amp-Pnp
authors W.Xu, A.Doshi, M.Lei, M.J.Eck, S.C.Harrison
compound source
Molecule: Tyrosine-Protein Kinase Src
Chain: Null
Fragment: Residues 86-836, Containing Sh2, Sh3, Kinase 2 Domains And C-Terminal Tail;
Synonym: C-Src, P60-Src
Ec: 2.7.1.112
Engineered: Yes
Biological_unit: Monomer
 Organism_scientific: Homo Sapiens
Organism_common: Human
Expression_system: Spodoptera Frugiperda Insect Cells
Expression_system_vector: Baculovirus
symmetry Space Group: P 21 21 21
R_factor 0.226
crystal
cell
length a length b length c angle alpha angle beta angle gamma
50.590 72.970 172.690 90.00 90.00 90.00
method X-Ray Diffractionresolution 1.5 Å
ligand PTR, ANPenzyme Protein-tyrosine kinase. Tyrosylprotein kinase. Protein kinase (tyrosine). Hydroxyaryl-protein kinase. Ectoprotein kinase. Gene lck protein kinase. Gene lck tyrosine kinase. Hydroxyaryl-protein kinase. Insulin receptor protein-tyrosine kinase. IRK. JAK protein tyrosine kinase. Protein p56lck kinase (phosphorylating):), protein (tyrosine) kinase (phosphorylating). Lck tyrosine kinase. Nonreceptor protein tyrosine kinase. P56lck protein tyrosine kinase. P56lck tyrosine kinase. P56lck protein kinase. P56lck kinase. Phosphotyrosyl-protein kinase. Protein p56c-lck kinase. Protein p56lck tyrosine kinase. Protein tyrosine kinase lck. Protein tyrosine kinase p56lck. Protein tyrosine kinase pp56lck. Protein kinase p56lck. Protein tyrosine kinase. Protein kinase (tyrosine-phosphorylating). Protein kinase p56-LCK. Protein kinase Lck. Receptor-activated Janus kinase. Tyrosine kinase lck. Tyrosine protein kinase p56lck. Tyrosine kinase p56lck. Tyrosine kinase. Tyrosine phosphokinase. Tyrosine protein kinase. Tyrosine-specific protein kinase. Tyrosylprotein kinase. V-fps protein-tyrosine kinase. Transferase E.C.2.7.1.112
related structures by homologous chain: 1F2F, 1FMK, 1QPC, 1SHF
similarity Src subfamily. Belongs to the tyr family of protein kinases. Contains 1 sh2 domain. Contains 1 SH3 domain.
catalytic activ. Atp + a protein tyrosine = adp + protein tyrosine phosphate.
post-translat. modifications The phosphorylated tail interacts with the sh2 domain thereby repressing kinase activity. Phosphorylated on tyr-529 by c-src kinase (csk).
genes V-SRC (A. sarcoma); V-SRC (A. sarcoma); SRC (G. gallus); V-ABL (F. sarcoma); ABL, ABL1, AGMX1, ATK, BEK, BPK, BTK, CSK, ERBB2, ERBB3, FGFBR, FGFR1, FGFR2, FLG, FLT2, FYN, GRB1, HCK, HER2, HER3, IGF1R, INSR, JTK7, KSAM, LCK, NEU, NGL, PIK3R1, SRC, SRC1, SYK (H. sapiens); NEF (H. immunodeficiency); NEF (H. immunodeficiency); ABL1, ABL, BLK, EFNB2, ELF2, EMT, EPHB2, EPL5, EPLG5, EPTH3, HTKL, ITK, LERK5, NUK, SEK3, TLK, TSK (M. musculus); CSK (R. norvegicus); V-SRC (R. sarcoma)
disease Leukemia,Philadelphia chromosome-positive,resistant to imatinib
Agammaglobulinemia,type 1,X-linked
XLA and isolated growthhormone deficiency
Antley-Bixler syndrome
Apert syndrome
Beare-Stevenson cutis gyrata syndrome
Craniofacial-skeletal-dermatologic dysplasia
Craniosynostosis,nonspecific
Crouzon syndrome
Gastric cancer,somatic
Jackson-Weiss syndrome
Pfeiffer syndrome
Saethre-Chotzen syndrome
Jackson-Weiss sydnrome
Kallmann syndrome 2
Ichthyosis vulgaris
Diabetes mellitus,insulin-resistant,with acanthosis nigricans
Leprechaunism
Rabson-Mendenhall syndrome
SCID due to LCK deficiency
Sialidosis,type I
Sialidosis,type II
Colon cancer,advanced
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (91 Kb) [Save to disk]
  • LPC: Ligand-Protein Contacts for 2SRC
  • CSU: Contacts of Structural Units for 2SRC
  • Likely Quarternary Molecular Structure file(s) for 2SRC
  • Retrieve 2SRC in mmCIF format [Save to disk]
    • View 2SRC in 3D
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    • Visual 3D analysis of 2SRC
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
    • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d2src_2, region 146-248 [rasmolscript] [script source]
        - Domain d2src_3, region 249-533 [rasmolscript] [script source]
        - Domain d2src_1, region 84-145 [rasmolscript] [script source]
  • Fold representative 2src from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [2src_]
  • SWISS-PROT database: [SRC_HUMAN]
  • Domain organization of [SRC_HUMAN] by SWISSPFAM
  • Domains found in 2SRC: [SH2] [SH3] [TyrKc ] by SMART
  • Conserved protein region description, domain view and alignment from family SH3 (PF00018) of Pfam.
  • Alignments of the sequence of 2SRC with the sequences similar proteins can be viewed for classification [SRC_HUMAN] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [SRC_HUMAN] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 2SRC
  • InterPro: IPR000980 , IPR001452 , IPR001245 , IPR000719
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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