4KBP Hydrolase (Phosphoric Monoester) date Oct 02, 1995
title Kidney Bean Purple Acid Phosphatase
authors T.Klabunde, N.Strater, R.Frohlich, H.Witzel, B.Krebs
compound source
Molecule: Purple Acid Phosphatase
Chain: A, B, C, D
Ec: 3.1.3.2
Other_details: 111kda Dimer Complex With Phosphate
 Organism_scientific: Phaseolus Vulgaris
Organism_common: Red Kidney Bean
symmetry Space Group: C 2 2 21
R_factor 0.192
crystal
cell
length a length b length c angle alpha angle beta angle gamma
132.700 347.300 128.700 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.7 Å
ligand NAG, FE, ZN, PO4enzyme Acid phosphatase. Acid phosphomonoesterase. Phosphomonoesterase. Glycerophosphatase. Acid monophosphatase. Acid phosphohydrolase. Acid phosphomonoester hydrolase. Uteroferrin. Acid nucleoside diphosphate phosphatase. Hydrolase E.C.3.1.3.2
note 4KBP is a representative structure
related structures by homologous chain: 1KBP
similarity Belongs to the Metallophos family.
subunit Homodimer; disulfide-linked.
catalytic activ. An orthophosphoric monoester + h(2)o = an alcohol + phosphate.
post-translat. modifications The c-terminus of the enzyme is often modified by the removal of four terminal residues.
subcellular loc. Secreted.
genes ACP1 (B. taurus); APPA, B0980 (E. coli); ACP1, ACPP (H. sapiens); ACP5 (S. scrofa); ACP5, ACPP (R. norvegicus)
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (326 Kb) [Save to disk]
  • LPC: Ligand-Protein Contacts for 4KBP
  • CSU: Contacts of Structural Units for 4KBP
  • Likely Quarternary Molecular Structure file(s) for 4KBP
  • Retrieve 4KBP in mmCIF format [Save to disk]
    • View 4KBP in 3D
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    • Visual 3D analysis of 4KBP
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
    • View 4KBP in 2D
  • Topology of domains/folds: Chain: A, Chain: B, Chain: C, Chain: D from TOPS (The Atlas of protein topology cartoons )
    • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Genome occurence of 4KBP's fold from GeneCensus
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d4kbpa2, region A:121-432 [rasmolscript] [script source]
        - Domain d4kbpa1, region A:9-120 [rasmolscript] [script source]
        - Domain d4kbpb2, region B:121-432 [rasmolscript] [script source]
        - Domain d4kbpb1, region B:9-120 [rasmolscript] [script source]
        - Domain d4kbpc2, region C:121-432 [rasmolscript] [script source]
        - Domain d4kbpc1, region C:9-120 [rasmolscript] [script source]
        - Domain d4kbpd2, region D:121-432 [rasmolscript] [script source]
        - Domain d4kbpd1, region D:9-120 [rasmolscript] [script source]
  • Fold representative 4kbp from FSSP and Dali (Families of Structurally Similar Proteins)
  • Domain Definition for 4kbp from 3Dee ( The Database of Protein Domain Definitions )
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [4kbp_A] [4kbp_B] [4kbp_C] [4kbp_D]
  • SWISS-PROT database: [PPAF_PHAVU]
  • Domain organization of [PPAF_PHAVU] by SWISSPFAM
  • Conserved protein region description, domain view and alignment from family Metallophos (PF00149) of Pfam.
  • Alignments of the sequence of 4KBP with the sequences similar proteins can be viewed for classification [PPAF_PHAVU] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [PPAF_PHAVU] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 4KBP
  • InterPro: IPR004843
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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