PSI-BLAST:
Since homology of the protein with other related sequence also provides broad range of the evolutionary information, therefore we have also developed PSI-BLAST module to predict subcellular localization of prokaryotic proteins. The performance of this module is poorer as compared to other modules developed in the present study. The SVM module based on this approach was able to predict the subcellular localization of the proteins with overall accuracy of 68%.
Hybrid based approach:
To enhance the prediction accuracy, we have devised methodologies to encapsulate more comprehensive information of a protein. A SVM-based module called as hybrid module was constructed on the basis of comprehensive information about the proteins including amino acid composition, dipeptide composition, composition of physico-chemical properties, and PSI-BLAST results.This module uses an input vector of 459 dimensions.The hybrid module was able to achieve a striking accuracy of 91%. The result confirmed that detection of subcellular localization of proteins requires wide range of information about a protein.
Output:
The output shows the input data as submitted by the user along with the prediction results. It gives the name ( if provided), input sequence, length of the sequence and prediction approach as used by the users. In addition to this different scores generated for all the four types of locations are also given. In case of hybrid approach, details such as RI value and expected accuracy are also displayed.