Our research focuses on infectious diseases, with a particular emphasis on the detection of waterborne pathogens such as Salmonella in clinical and environmental settings. We investigate virulence-associated biomarkers and invention based peptides, enabling advances in both diagnostics and vaccine development. Our work involves the generation of high-affinity antibodies and aptamers (synthetic recognition elements) targeting clinically relevant markers. We study fundamental aspects of interactions between enzymes such as Urease, Lipase, β-Galactosidase etc. and nanomaterial-based inhibitors. We have developed a landmark ultrasensitive, cost-effective, and user-friendly biosensing platform based on enzyme-metal interplay, representing a significant step toward accessible point-of-care diagnostics. In addition, our research extends to bioconjugation strategies and small-molecule immunodetection, supporting a broad range of translational applications.

• Bharti, P. Bhardwaj, B. Bisht, R. Bala, Sagrika, M. Rohit, J. Singh, V. Bhalla, Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers, ACS Applied Materials & Interfaces, 18 (2026) 12423-12431.
• P. Bhardwaj, B. Bisht, V. Bhalla, Nanoscale size impact of nanoparticle interaction and activity studies with urease, Nanoscale, 17 (2025) 5000-5004.
• B. Bisht, P. Bhardwaj, S. Chauhan, Sagrika, Vedika, N. Basnal, V. Bhalla, Nanocatalysis of silver-nanobioprobe based supersensitive electrochemical detection of Salmonella serotypes targeting virulence protein, Biosensors and Bioelectronics, 268 (2025) 116872.
• M. Choudhary, B. Bisht, J.K. Saini, Bharti, P. Singh, P. Bhardwaj, R. Dilawari, A.K. Pinnaka, P. Ray, M. Gupta, S. Sethi, C.R. Suri, M. Raje, V. Bhalla, Bifunctionalized nanobioprobe based rapid color-shift assay for typhoid targeting Vi capsular polysaccharide, Biosensors and Bioelectronics, 228 (2023) 115195.
• S. Kakkar, S. Chauhan, Bharti, M. Rohit, V. Bhalla, Conformational switching of aptamer biointerfacing graphene-gold nanohybrid for ultrasensitive label-free sensing of cardiac Troponin I, Bioelectrochemistry, 150 (2023) 108348..

• 201811035461; A pH based colorimetric bioassay method for bacterial detection in water by naked eye.
• 202111043591; DNA aptamers directed against cardiac biomarker peptides.