Principal Scientist:

Dr. Amit Kumar

Contact Address



Cell Biology, Genome Integrity, Autophagy, and Mechanobiology.


Genome integrity is precisely regulated in all organisms and DNA damage response (DDR) proteins are major players in this process. Mutation in DDR proteins cause developmental defects in patients that might relate to altered nuclear structure, chromatin architecture in physiological conditions .Environmental, exogenous/endogenous agents continuously damage human genome. The toxic effects of these factors induce lesions in DNA causing mutations and deletions in chromosomes resulting in genome instability that have immediate effect on cell function as well as long-term consequences. To counteract the deleterious effects of DNA lesions, cells have developed sophisticated genome caretaking mechanisms to overcome DNA damage through specialized network of DNA repair pathways regulated by DNA damage checkpoint proteins. These apical DNA damage checkpoint proteins regulate integrity of chromosomes through coordination of the cell cycle progression with DNA repair, DNA replication and recombination processes through induction of the DNA damage response (DDR). Improper repair of DSBs can lead to various developmental defects and become genesis of many modern diseases. Overall, we aim to investigate the canonical and non-canonical DNA damage checkpoint proteins function in maintenance of cell physiology in coordination with other cell processes including Autophagy (through distributing mechanical cues, maintainance of stem cell potency, coordinating DNA damage with transcriptional response) in response to changes in chromatin dynamics through various unknown mechanisms. Editorial Board : Mutagenesis Journal (Oxford University Press, U.K.) Ecancer medicalscience, U.K.

Major Publications

  • Yadav V. et al., HSc70 interactome reveal major role of macroautophagy and minor role of chaperone mediated autophagy in K-Ras G12V cell proliferation and survival. Journal of Proteomics. 2022.
  • Awasthi P. et al., Proteome profiling of phosphatidylinositol-5-phosphate 4-kinase type 2A and 2B knockdown cells identify modifications in key regulators involved in cell homeostasis and genome integrity. Genome Instability & Disease. 2022.
  • Kosar M. et al., Human nucleoporin Tpr coordinates mRNA processing with chromosome replication. Nature Communication. 2021 .
  • Kidiyoor GR. Et al., ATR preserves cell mechanics and prevents nuclear collapse during interstitial migration. Nature Communication. 2020.
  • Kidiyoor Gret al., ATR-mediated regulation of nuclear and cellular plasticity. DNA Repair (Amst). 2016.
  • Awasthi P, et al. ATM and ATR signaling at a glance. J Cell Sci. 2015.

Lab Members

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