Protein Engineering, Post-translational modification analysis, Mass spectrometry, Directed evolution.
Our group holds interest in tinkering with and exploring the post-translational modifications of proteins namely glycosylation and N- α acetylation, in bacteria. At CSIR-IMTECH, we have successfully developed methods for protein O- and S- glycosylation and currently we are endeavouring to develop a versatile tool-kit for efficient glycosylation/acetylation of proteins or peptides for useful applications. A parallel research interest of our group is to understand the biological significance of these modifications in cellular context, more specifically in M. tuberculosis pathogenesis. In sync with CSIR-Spirit, we actively collaborate with industry and have ongoing association with partners namely Revelations Biotech Pvt. Ltd., and Premas Biotech Pvt. Ltd. In past, our association with Criyagen Agri and Biotech Pvt. Ltd. led to a successful market launch of a growth promoting product for crop applications.