Molecular Biology, Protein Engineering & Biotherapeutics.
My expertise in biology ranges from biotechnology to genetic manipulation of bacterial cells to acquire desired phenotypes, to genetic engineering of proteins, to transgenic animal models, to cell and tissue engineering. The current focus of my laboratory is to develop novel therapeutic proteins. We have been able to develop the biosimilar of G-CSF (Filgrastim). Additionally, we are also developing the PEGylated G-CSF biosimilar (Pegfilgrastim), which has longer half-life and is administered once per cycle of chemotherapy. Recently, my laboratory has also embarked upon development of advanced G-CSF molecules. We work on process development for efficient protein purification and characterization of this important therapeutic protein. Besides, G-CSF, we also strive to engineer novel and valuable cytokines, growth factors and antibodies. Given that a number of therapeutic proteins are imported, the creation of biosimilars and biobetters of many of the biotherapeutics is the need of the hour in India. We are also interested in development of animal models for various diseases and their deployment in analysis of efficacy of the lab-made therapeutics. Another interest of the laboratory is to decipher the role of vital organ microbiome in health and diseases.
- Datta S, Alam MP, Majumdar SS, Mehta AK, Maiti S, Wadhwa N, Brahmachari V. Nucleosomal occupancy and CGG repeat expansion: a comparative analysis of triplet repeat region from mouse and human fragile X mental retardation gene 1. Chromosome Research. 2011 May; 19(4), pp 445-455.DOI https://doi.org/10.1007/s10577-011-9206-7.
- Alam MP*,Datta S*, Majumdar S, Mehta AK, Baskaran S, Gulati N, Brahmachari V. Comparative analysis of DNA methylation in transgenic mice with unstable CGG repeats from FMR1gene.Epigenetics. 2010 April; 5(3),pp241-248.(*Co-first author DOI https://doi.org/10.4161/epi.5.3.11417.
- V.Hoenerhoff MJ, Chu I, Barkan D, Liu ZY, Datta S, Dimri GP, Green JE. BMI1 cooperates with H-RAS to induce an aggressive breast cancer phenotype with brain metastases. Oncogene. 2009 June; (28)34,pp 3022-3032.DOIhttps://doi.org/10.1038/onc.2009.165.
- Datta S, Hoenerhoff MJ, Bommi P, Sainger R, Guo WJ, Dimri M, Band H, Band V, Green JE, Dimri GP. Bmi-1 cooperates with H-Ras to transform human mammary epithelial cells via dysregulation of multiple growth-regulatory pathways. Cancer Research. 2007 Nov.;67(21), pp 10286-95. DOIhttps://doi.org/10.1158/0008-5472.CAN-07-1636.
- Guo WJ, Datta S, Band V, Dimri GP. Mel-18, a polycomb group protein, regulates cell proliferation and senescence via transcriptional repression of Bmi-1 and c-Myconcoproteins. Molecular Biology of the Cell.2006 Dec.; 18(2), pp 536-46. DOI https://doi.org/10.1091/mbc.e06-05-0447.