Emerging and re-emerging viruses are posing serious public health problem globally including India with the huge economic loss. The World has witnessed several epidemics and outbreaks of many viruses in the recent past e.g. Zika, Ebola, Influenza, dengue, chikungunya, Japanese encephalitis, hepatitis and respiratory syncytial virus etc. It is important to decipher the Indian virome in the clinical/other settings to better understand their different aspects. Importantly, dengue remains a medical problem in India and tropical part of the world affecting 390 million people per annum (http://www.who.int/mediacentre/factsheets/fs117/en/). There is an urgent medical need to develop antiviral therapeutics against dengue for which no drug or effective vaccine is presently available. To address these unmet medical needs, CSIR-IMTECH Virology Discovery Unit is planning to find a solution in mission mode. We are focusing on the development of antiviral therapeutics against dengue/flavivirus targeting host/viral factors. We are also exploring clinically or medically important Indian virome using NGS metagenomics approach for better diagnostics.